What makes good cytoplasm




















The plasma membrane is the outer lining of the cell. It separates the cell from its environment and allows materials to enter and leave the cell. These organelles can float freely in the cytoplasm or be connected to the endoplasmic reticulum see above. Other chapters in Help Me Understand Genetics. Genetics Home Reference has merged with MedlinePlus. Learn more. The information on this site should not be used as a substitute for professional medical care or advice.

Contact a health care provider if you have questions about your health. What is a cell? From Genetics Home Reference. Human cells contain the following major parts, listed in alphabetical order: Cytoplasm Within cells, the cytoplasm is made up of a jelly-like fluid called the cytosol and other structures that surround the nucleus.

Endoplasmic reticulum ER This organelle helps process molecules created by the cell. This page has been archived and is no longer updated. Like mitochondria, chloroplasts likely originated from an ancient symbiosis, in this case when a nucleated cell engulfed a photosynthetic prokaryote. Indeed, chloroplasts resemble modern cyanobacteria, which remain similar to the cyanobacteria of 3 million years ago. However, the evolution of photosynthesis goes back even further, to the earliest cells that evolved the ability to capture light energy and use it to produce energy-rich molecules.

When these organisms developed the ability to split water molecules and use the electrons from these molecules, photosynthetic cells started generating oxygen — an event that had dramatic consequences for the evolution of all living things on Earth Figure 1. Figure 1: The origin of mitochondria and chloroplasts Mitochondria and chloroplasts likely evolved from engulfed prokaryotes that once lived as independent organisms.

At some point, a eukaryotic cell engulfed an aerobic prokaryote, which then formed an endosymbiotic relationship with the host eukaryote, gradually developing into a mitochondrion.

Eukaryotic cells containing mitochondria then engulfed photosynthetic prokaryotes, which evolved to become specialized chloroplast organelles.

Today, chloroplasts retain small, circular genomes that resemble those of cyanobacteria, although they are much smaller. Mitochondrial genomes are even smaller than the genomes of chloroplasts. Coding sequences for the majority of chloroplast proteins have been lost, so these proteins are now encoded by the nuclear genome, synthesized in the cytoplasm, and transported from the cytoplasm into the chloroplast. Like mitochondria, chloroplasts are surrounded by two membranes.

The outer membrane is permeable to small organic molecules, whereas the inner membrane is less permeable and studded with transport proteins.

The innermost matrix of chloroplasts, called the stroma, contains metabolic enzymes and multiple copies of the chloroplast genome. Chloroplasts also have a third internal membrane called the thylakoid membrane, which is extensively folded and appears as stacks of flattened disks in electron micrographs. The thylakoids contain the light-harvesting complex , including pigments such as chlorophyll, as well as the electron transport chains used in photosynthesis Figure 2.

These components are organized into three major layers: the primary cell wall, the middle lamella, and the secondary cell wall not pictured. The cell wall surrounds the plasma membrane and provides the cell tensile strength and protection. Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol. Nature Reviews Genetics 9, All rights reserved. The primary cell wall is just outside the plasma membrane. Cellulose is the major component of the primary cell wall.

Cellulose microfibrils are represented as large, green cylinders that lie on top of one another in a crisscross fashion. Among the cellulose microfibrils are pectin molecules, which are depicted as long, thin, orange strands that curve and have some squiggled sections. The primary cell wall also contains hemicellulose, which is represented by dark-green, thin lines that are straight with some sharp turns, and soluble protein, which is depicted as small blue and green spheres.

The middle lamella forms the outermost layer and is depicted as a flat, translucent, blue slab. Many pectin molecules are shown inside the middle lamella.

In this layer, the pectin molecules are represented as light-green strands with some curvature. The middle lamella also contains soluble proteins similar to those in the primary cell wall. Crowding effects on diffusion in solutions and cells. Annu Rev Biophys. Models of macromolecular crowding effects and the need for quantitative comparisons with experiment. Curr Opin Struct Biol. Fractional-time random walk subdiffusion and anomalous transport with finite mean residence times: faster, not slower.

Structure of metaphase chromosomes: a role for effects of macromolecular crowding. PloS One. Macromolecular crowding remodels the energy landscape of a protein by favoring a more compact unfolded state. J Am Chem Soc.

Beyond oil and water—phase transitions in cells. High-resolution multi-dimensional NMR spectroscopy of proteins in human cells.

Down to atomic-scale intracellular water dynamics. EMBO Rep. Ancient ubiquitous protein-1 mediates sterol-induced ubiquitination of 3-hydroxymethylglutaryl CoA reductase in lipid droplet-associated endoplasmic reticulum membranes. Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels. Phase transitions in the assembly of multivalent signalling proteins. Dynamic microcompartmentation in synthetic cells. Cytoarchitecture and physical properties of cytoplasm: volume, viscosity, diffusion, intracellular surface area.

Int Rev Cytol. Size dependence of protein diffusion in the cytoplasm of Escherichia coli. J Bacteriol. Subcellular localization of RNA and proteins in prokaryotes. Trends Genet. What is the true enzyme kinetics in the biological system? An investigation of macromolecular crowding effect upon enzyme kinetics of glucosephosphate dehydrogenase. Biochem Biophys Res Commun.

Dynamic reorganization of metabolic enzymes into intracellular bodies. Annu Rev Cell Dev Biol. Cell water dynamics on multiple time scales. Femtosecond dynamics of intracellular water probed with nonlinear optical Kerr effect microspectroscopy.

Biophys J. Wanted: a positive control for anomalous subdiffusion. Macromolecular crowding fails to fold a globular protein in cells. Cytoplasmic water and hydration layer dynamics in human red blood cells. Lipid droplets and cellular lipid metabolism. Annu Rev Biochem. Exploring weak, transient protein—protein interactions in crowded in vivo environments by in-cell nuclear magnetic resonance spectroscopy.

Macromolecular crowding and protein stability. Getting RNA and protein in phase. From protoplasmic theory to cellular systems biology: a year reflection. Am J Physiol Cell Physiol.

The structure of protoplasm.



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